10 year old paper showing brain proteome changes 1 min postmortem!
10 year old paper showing brain proteome changes 1 min postmortem! Ive got a ton of new cool papers on my desktop to read when I get caught up, but I landed on this study while looking for something else, and I found the results remarkable. The questions: 1) How much does postmortem degradation effect the brain proteome? To go after the answer, this team used a mouse model system. What they found (using 2D-DIGE, and LTQ and a TOF) is some massive changes even 1 minute after sacing the mice. 2) Can they find markers that would give them a reliable measurement of the quality of the brains for use in further analysis? I found myself multiple times reading between the lines and imagining that this group primarily worked with human postmortem tissue and that there was a lot of variability in how those people had died and how long it was before the samples would find the way to their labs. What they found is really interesting -- almost immediately changes after death if they dont do so...